pyrene labelled muscle actin (Cytoskeleton Inc)

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Cytoskeleton Inc pyrene labelled muscle actin
Pyrene Labelled Muscle Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/skeletal+muscle+actin/Actin+protein+pyrene+labeled+rabbit+skeletal+muscle/bio_rxiv__64898__2026__05__16__725640-220-0-3
Average 95 stars, based on 113 article reviews

pyrene labelled muscle actin - by Bioz Stars, 2026-09

95/100 stars

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Purification:

Article Title: Neutrophil Recruitment to the Lung in both C5a and CXCL1-Induced Alveolitis is Impaired in Vitamin D Binding Protein Deficient Mice
Article Snippet: Chicken anti-human DBP antibody was purchased from Gallus Immunotech (Cary, NC). .. Highly purified skeletal muscle actin was obtained from Cytoskeleton, Inc. (Denver, CO) and type I collagen from Elastin Products, Inc. (Owensville, MO). .. Goat anti-actin (I-19) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and pan anti-actin mAb ACTN05 was purchased from Thermo Scientific/Lab Vision (Kalamazoo, MI).

Article Title: Structural details and mechanism of filamentous actin organization by the isoforms vinculin and metavinculin
Article Snippet: .. Skeletal muscle actin was either available in our laboratory or bought from Cytoskeleton Inc. Vt was purified as described in chapter III. ..

Article Title: Nesprin interchain associations control nuclear size
Article Snippet: .. Purified skeletal muscle actin (Cytoskeleton, #AKL99) was resuspended in G-buffer (0.2 mM CaCl 2 , 0.2 mM ATP, 0.5 mM DTT, 5 mM Tris, pH 8.0), incubated for 1 h on ice and subjected to centrifugation at 30,000 g for 15 min at 4 °C, to remove actin polymers. .. His-tagged nes-2 ABD was purified as described above, concentrated using Pierce protein concentrators (20K MWCO; Thermo Scientific), and dialyzed overnight at 4 °C in P-buffer (2 mM MgCl 2 , 50 mM KCl, 1 mM ATP, 0.5 mM DTT, 5 mM Tris, pH 8.0) containing protease inhibitors.

Concentration Assay:

Article Title: A nebulin super-repeat panel reveals stronger actin binding toward the ends of the super-repeat region.
Article Snippet: Introduction: Nebulin is a giant actin-binding protein in the thin filament of the skeletal muscle sarcomere.. Studies of nebulin interactions are limited by the size, complexity, and poor solubility of the protein.. We divided the nebulin super-repeat region into a super-repeat panel, and studied nebulin/actin interactions.

Centrifugation:

Binding Assay:

Article Title: Actin activates Pseudomonas aeruginosa ExoY nucleotidyl cyclase toxin and ExoY-like effector domains from MARTX toxins
Article Snippet: .. 12.5 μg of ExoY-FH, skeletal muscle actin from rabbit (AKL99, Cytoskeleton, Inc., designated in the text by MA-99), or both proteins in 450 μl of binding buffer [50 mM Na-phosphate pH 8.0, 300 mM NaCl, 20 mM imidazole, 0.01 % triton X-100, complete EDTA-free protease inhibitor cocktail (Roche)] were allowed to bind in batch to 5 μl Ni-NTA agarose (Quiagen) for 1 h at 4 °C rotating in Durapore filter units (Millipore). ..

Protease Inhibitor:

Incubation:

Labeling:

Article Title: Dynamic stabilization of actin filaments
Article Snippet: .. Skeletal muscle actin, prepared as described above, was mixed with pyrene actin (AP05; Cytoskeleton) and diluted in G buffer (60 μM 10% pyrene labeling). ..

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